Stellenmarkt

 

 


 

 

A PhD position is available at the Department of Parasitology, Center of Infectious Diseases, University of Heidelberg Medical School.


PhD in molecular Parasitology and Cell biology

Introduction: Our group works on the interaction of the malaria parasite with its chosen host cell, the mature human erythrocyte. The group is funded by the German Research Foundation, and currently composes four PhD student, the group leader (Jude Przyborski) and rotating bachelor/master and medical students. We are situated in the Department of Parasitology at the University of Heidelberg Medical School. The Department consists of 2 Professors and 8 groups which all work on the biology of the malaria parasite, and we are thus ideally situated to offer an intellectually stimulating and supportive scientific environment. Heidelberg itself is a lovely town (especially in the summer), and is home to the oldest and one of the most respected Universities in Germany (https://www.klinikum.uni-heidelberg.de/Malaria-9-Przyborski.143168.0.html).

Additional training opportunities: The selected student will be encouraged to enter the HBIGS graduate school programme which offers numerous opportunities for additional training (http://www.hbigs.uni-heidelberg.de).

Project Title: Atypical protein transport in malaria-infected erythrocytes: How does a membrane protein “jump” across membranes?

Project leader: PD Jude Przyborski

Application Deadline: 28 February 2019

Start of PhD project: ASAP

Source of Funding: DFG

Level of funding: Funding is available at E13/65% level for 3 years

Application: Please send your CV, statement of motivation and contact details for three referees by email to Diese E-Mail-Adresse ist gegen Spambots geschützt! JavaScript muss aktiviert werden, damit sie angezeigt werden kann. . Alternatively, you can apply via the HBIGS online system (http://www.hbigs.uni-heidelberg.de).

Project Description: Malaria is still one of the most important infectious diseases infecting humans, and kills about 400, 000 people annually. For this reason, it is essential to understand more about the cell and molecular biology of this parasite. Earlier studies on the basic cell biology of this parasite have revealed a multitude of novel phenomenon, including many related to how the parasite traffics proteins to various structures (Przyborski et al, 2016). This project aims to address one particular phenomenon, the transport of proteins beyond your boundary of the parasite’s plasma membrane. A previous study from our lab has uncovered an unusual pathway for trafficking of parasite proteins in this way, and this current project aims to build on our experience and further understand this process (Tribensky et al, 2017).

The human malaria parasite P. falciparum invades and propagates within mature red bloods cells. During invasion, the parasite envelops itself within a so-called parasitophorous vacuolar membrane (PVM), which acts as an interface between parasite and host cell, and is implicated in a number of processes important for parasite propagation. To this end, the parasite synthesises a number of proteins which intercalate into the PVM and are essential for parasite survival. However, it is so far unclear how exactly these proteins are both trafficked and sorted from the parasite to the PVM. In this project, using a number of complementary techniques, we aim to address this question and elucidate both the signals and mechanisms utilised to enable protein transport to the PVM. As a model protein, we shall firstly dissect the sorting signals and mechanisms required for the transport of the Exported Protein 1 to the PVM. Principles uncovered during this initial study will then be used to study the trafficking of further PVM-resident proteins such as members of the ETRAMP family. As protein transport to the PVM is a process underlying intra-erythrocytic development of the parasite and the subsequent pathology in the human host, any novel pathways may prove to be a chink in the parasite’s armour which may later be targeted for development of anti-parasitic drugs.

References:

Przyborski, J.M., Nyboer, B. & Lanzer, M. (2016) Ticket to ride: export of proteins to the Plasmodium falciparum-infected erythrocyte. Mol Microbiol, 101, 1-11.

Tribensky, A., Graf, A.W., Diehl, M., Fleck, W. & Przyborski, J.M. (2016) Trafficking of PfExp1 to the parasitophorous vacuolar membrane of Plasmodium falciparum is independent of protein folding and the PTEX translocon. Cell Microbiol, 19

Methods that will be used:

Molecular biology, protein biochemistry, proteomics, cell culture, transfection, cell-based assays.

Profile of candidate’s qualification:

Candidates should have a masters degree in molecular cell biology, biochemistry or equivalent disciplines. Experience with cell culture, protein biochemistry and proteomics would be an advantage. In addition, excellent knowledge in English (speaking, reading, writing) is required. The candidate should be able to independently solve problems and have a willingness to learn new techniques, including culture and transfection of malaria parasites, cell-based assays and protein purification.

Keywords:

Malaria, protein transport, erythrocytes, membrane transport.

 

 


 

 

A PhD position is available at the Institute of Animal Pathology, University of Bern to join our molecular parasitology group.

PhD Position - Molecular Parasitology

Our lab works on the host pathogen interaction of intracellular parasites (www.itpa.vetsuisse.unibe.ch/research/host_pathogen_interactions/group_olias/index_eng.html)
We study the mechanisms of host cell transformation by apicomplexan parasites. As a main model organism, we use the cattle pathogen Theileria annulata. Infection of bovine leukocytes with this eukaryotic parasite causes cellular transformation, and infected cells share many hallmarks with cancer. Theileria-transformed cells proliferate continually, become resistant to apoptosis, and acquire a metastatic phenotype. Theileria is the only eukaryotic pathogen known to transform its host cell, and thus Theileria infection provides a unique and reversible model with which to study signalling pathways associated with transformation and cancer. By identifying host factors that are essential for Theileriadriven transformation, we expect not only to discover potential new drug targets for Tropical Theileriosis, but also to uncover novel pathways that could be involved in cancer.

In the scope of this project, we will use a newly engineered bovine genome wide single guide RNA library to perform large scale genome wide CRISPR/Cas9 knock out screens. By comparing host factors that are essential for the survival of Theileria-infected cells, but not in their non-infected counterparts, we will identify host factors and signaling pathways that are specifically required for parasite-mediated transformation. Our institute has well established expertise in the use of genome-wide screening technology (www.itpa.vetsuisse.unibe.ch/research/therapy_escape_of_cancer/index_eng.html), and in this exciting project we will apply these cutting edge techniques to bovine cells. Next, we will perform robust validation experiments including knock out and inducible shRNA depletion of candidates. The in-depth study of selected host pathways will involve a variety of advanced biochemical, cellular, proteomic and genetic approaches to better understand host cell transformation caused by the parasite.

We are looking for a highly motivated student with a strong interest in cell biology to join our group. Experience in molecular biology and biochemistry is an advantage. The position requires a degree in biology, (veterinary) medicine, biomedical sciences, biochemistry or a related field. Fluent spoken and written English are prerequisites for this position.

We offer a young and enthusiastic research group, an inspiring research environment, state-ofthe art research facilities at the attractive working location in Bern. The remuneration is in accordance with the Swiss National Science Foundation (SNF) regulations. The PhD student will be enrolled in the Graduate School of Cellular and Biomedical Sciences (http://www.gcb.unibe.ch)
at the University of Bern.

The position is available as of January 1st 2019 or by agreement.

How to apply: Please send your application, which should include a motivation letter, CV and references (letters / contact information of three references by email to: Ms. Christine Herzig, Diese E-Mail-Adresse ist gegen Spambots geschützt! JavaScript muss aktiviert werden, damit sie angezeigt werden kann. ). For questions regarding the project please contact Dr. Philipp Olias ( Diese E-Mail-Adresse ist gegen Spambots geschützt! JavaScript muss aktiviert werden, damit sie angezeigt werden kann. ).
Applications will be accepted until the vacancy is filled.



 


 

 

 

The Bernhard Nocht Institute (www.bnitm.de) is the largest Research Institute for Tropical Medicine in Germany and is the National Reference Centre for Tropical Pathogens, a WHO Collaborating Centre and member of the Leibniz Research Association.

The Bernhard Nocht Institute for Tropical Medicine (BNITM) in Hamburg currently has an opening for a

PhD Student (f/m/d) in the Leishmaniasis Research Group

The Leishmaniasis Research Group, headed by Dr. J. Clos, investigates the mechanisms of Leishmania life cycle control, in particular the role of chaperone proteins in the process of stressinduced stage conversion. Other topics are the mechanisms of drug resistance and the advancement of new therapeutic compounds against leishmaniasis. For further information see: https://www.bnitm.de/forschung/forschungsgruppen/molekularbiologie-undimmunologie/arbeitsgruppe-clos/

Your tasks:

The successful candidate will analyse the mechanisms of regulated protein synthesis in the context of HSP90-dependent signal transduction, using biochemical, cell biology and next generation sequencing technologies in combination with reverse and complementation genetic approaches.

Your profile:

  • We are looking for candidates holding a master's degree in biochemistry, biomedical sciences or related fields with established experimental skills in molecular cloning, protist cell biology and/or systems biology.
  • Given the international composition of the group, good English language skills, written and oral, are required.
  • Computer literacy is expected.
  • A basic knowledge of bioinformatic principles will be advantageous.

The BNITM offers a diversified training in all aspects of infection biology, but also in soft skills.The position is available immediately and the studentship is targeted for a 3-year period.
Remuneration is in accordance with German salary scheme for the public service (TV-AVHHamburg). We support our employees in achieving a work-life balance and promote the
professional equality of women and men. We strive to assist women in their scientific career, increase the number of women in research and reduce underrepresentation
in all areas and positions in general. Applicants with disabilities willbe given preference if equally qualified.


Please send your application (incl. motivation letter, CV, list of publications, references) referring to “PhD student - Leishmaniasis Research Group” until 30.11.2018 to Ms. Dvorak
( Diese E-Mail-Adresse ist gegen Spambots geschützt! JavaScript muss aktiviert werden, damit sie angezeigt werden kann. ) or via postal mail to Bernhard Nocht Institute for Tropical Medicine, HR Department, Bernhard-Nocht-Straße 74, D-20359 Hamburg.
For further questions please contact Dr. J. Clos per email at Diese E-Mail-Adresse ist gegen Spambots geschützt! JavaScript muss aktiviert werden, damit sie angezeigt werden kann. .

 

 


 

 

The Breloer Group "Helminth Immunology"
at the Bernhard Nocht Institute for Tropical Medicine (BNITM) in Hamburg
invites applications for the position of a

Postdoctoral researcher (m/f)

We offer a 3-years position, starting in February 2019 with the option of a 3-years extension (6 years in total). The payment and benefits are based on the TV-AVH salary scale.
Parasitic worms or helminths infect one-third of the human population. They manage to survive in immune competent hosts sometimes for decades, by actively dampening the immune response of their hosts. The Research Group "Helminth Immunology" exploits mouse models of intestinal and tissue dwelling helminth parasites to study

i) initiation and execution of innate and adaptive immune response against helminths
ii) helminth-induced countermeasures i.e. immune modulation and immune evasion
iii) the consequence of concurrent helminth infection on the immune response to third party antigens in situations of vaccination or co-infection

We are looking for an enthusiastic and highly motivated scientist who is passionate about immunology and parasitology and pursuing a scientific career to join our team. The BNITM offers a vivid and collaborative environment for research that is equipped with state-of-the-art facilities for parasitological and immunological research. The applicant will be involved in ongoing projects and also encouraged to develop independent research projects in the frame of the Helminth Immunology Group. For further information see: https://www.bnitm.de/en/research/research-groups/molecular-biology-and-immunology/breloer-group/
The applicant must hold a PhD or Dr. rer. nat. in biology, biochemistry, molecular biology or a related subject. A strong background in immunology and techniques related to infection immunology in mouse and/or human systems is advantageous. Excellent command of written and spoken English is required; German language skills are advantageous but not mandatory. Experience as postdoctoral researcher and/or experience with independent development of research projects, record of publications and/or grants will be favourable.
We support our employees in achieving a work-life balance and promote the professional equality of women and men. We strive to assist women in their scientific career, increase the number of women in research and reduce under-representation in all areas and positions in general. Applicants with disabilities will be given preference when equally qualified.
For queries regarding this position, please contact PD Dr. Minka Breloer ( Diese E-Mail-Adresse ist gegen Spambots geschützt! JavaScript muss aktiviert werden, damit sie angezeigt werden kann. )
To apply please send your application with CV, publication list, contact detail of 2-3 referees and brief outline of your research interests within the Helminth Immunology Group referring to “PostDoc Helminth-Immunology” until 01.12.2018 as a single PDF via email to Ms. Linda Dvorak: Diese E-Mail-Adresse ist gegen Spambots geschützt! JavaScript muss aktiviert werden, damit sie angezeigt werden kann.

 

 


 


PhD student position

Max Planck Institute for Developmental Biology, Tübingen

Department "Integrative Evolutionary Biology" (director Dr. Ralf J. Sommer)

Research group "Parasitic nematodes" (group leader Dr. Adrian Streit)

 

A PhD student position is available immediately in the research group of Dr. Adrian Streit (http://www.sommerlab.org/research/parasitic-nematodes.html) in the department for Integrative Evolutionary Biology (http://www.sommerlab.org/home.html) at the Max Planck Institute for Developmental Biology in Tübingen, Germany.

Topic: Comparative studies of fat storage and nutritional state dependent life history switches in parasitic nematodes of the family Strongyloididae and the free-living nematodes Prisitonchus pacificus and Caenorhabditis elegans.

The candidate should have or expect to obtain in the near future a Master's degree in Biology or a related topic. A solid knowledge of genetics and molecular biology is expected, previous experience with nematodes is not required. The working language in the department is English, knowledge of German is not required. The successful candidate will be enrolled in the institute's international PhD program and must therefore meet all requirements for admission to this program. For more information about these requirements and the program in general please see http://phd.eb.tuebingen.mpg.de.

Applications will be considered until the position is filled. All applications arriving before November 10th 2018 will be given equal consideration.

To apply, please send by Email a cover letter with a statement of interests, a brief summary of your Master thesis, a CV and the names and contact addresses of two academic references to

Dr. Adrian Streit
Max Planck Institute for Developmental Biology
Max-Planck-Ring 9
72076 Tübingen, Germany
Diese E-Mail-Adresse ist gegen Spambots geschützt! JavaScript muss aktiviert werden, damit sie angezeigt werden kann.

Short listed candidates will be invited by the PhD program office to submit full applications with all the documents.

Zuletzt aktualisiert am Mittwoch, den 12. Dezember 2018 um 12:31 Uhr