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A PhD position is available at the Institute of Animal Pathology, University of Bern to join our molecular parasitology group.

PhD Position - Molecular Parasitology


Our lab works on the host-pathogen interaction of intracellular parasites of human and veterinary importance. We study the mechanisms of host cell manipulation by the related apicomplexan
parasites Cryptosporidium and Theileria. One of our main interests is to better understand the interference of parasite effector proteins with host signaling pathways.

Cryptosporidium parasites infect the intestinal epithelium of humans and animals and cause a severe diarrheal disease. The zoonotic potential of Cryptosporidium transmitted from animals is of great
importance in human medicine and the high mortality of children infected with Cryptosporidium in less industrialized regions has intensified the research over the past few years. Recent scientific
breakthroughs in CRISPR genome editing and organoid culture systems now enable us to study the molecular biology of this parasite. Despite the high importance of cryptosporidiosis, there is a lack of
therapeutics and a better understanding of the host-pathogen interaction is urgently needed.

In the scope of this project, we intend to develop a variety of advanced biochemical, proteomic and genetic approaches to better understand the Cryptosporidium-induced host cell manipulation and
pathogenesis that causes diarrheal disease. We are particularly interested in identifying secreted Cryptosporidium effector proteins and in defining their mode of action. The project is likely to directly
advance the goal of validating new essential and parasite-specific targets, and this will support future efforts to develop new treatments against cryptosporidiosis.

We are looking for a highly motivated student with a strong interest in cell biology to join our group.
Experience in molecular biology and biochemistry is an advantage. The position requires a degree in biology, veterinary or human medicine, biomedical sciences, biochemistry or a related field. Fluent
spoken and written English are prerequisites for this position.

We offer a young and enthusiastic research group, an inspiring research environment, state-of-the art research facilities at the attractive working location in Bern. The remuneration is in accordance with the Swiss National Science Foundation (SNF) regulations www.snf.ch. PhD students will be enrolled in the Graduate School of Cellular and Biomedical Sciences www.gcb.unibe.ch at the University of Bern.
The position is available as of March 1st, 2019 or by agreement.

How to apply: Please send your application as a single pdf document including a motivation letter, CV and references (letters / contact information of two references) by email to Christine Herzig
Diese E-Mail-Adresse ist gegen Spambots geschützt! JavaScript muss aktiviert werden, damit sie angezeigt werden kann. . For further questions regarding the project please contact Philipp Olias www.itpa.vetsuisse.unibe.ch/research/host_pathogen_interactions/group_olias/index_eng.html.







Naturwissenschaftliche Promotionsstelle im Bereich Infektionsbiologie


In der Abteilung für Zelluläre und Angewandte Infektionsbiologie der RWTH Aachen University ist zum 1. Mai 2019 eine naturwissenschaftliche Promotionsstelle zu besetzen.

Unser Profil:

Unsere Arbeitsgruppe beschäftigt sich mit der Zellbiologie des humanpathogenen Malariaerregers Plasmodium falciparum. Im Besonderen untersuchen wir die Signalwege in den Sexualstadien des Parasiten, die für die die Übertragung der Malaria verantwortlich sind und wichtige Zielstrukturen für Chemotherapeutika darstellen. Der Schwerpunkt unsere Abteilung liegt in den Bereichen der molekularen Parasitologie und der medizinischen Mikrobiologie. Wir bieten ein dynamisches und internationales Umfeld in einem aktuellen und interdisziplinären Forschungsgebiet.

Ihr Profil:
Sie haben ein erfolgreich abgeschlossenes Masterstudium mit biologischem Hintergrund und besitzen Erfahrungen in der Zell- und Molekularbiologie sowie der Biochemie. Sie sind flexibel und engagiert und haben Freude an eigenständiger wissenschaftlicher Arbeit in einem internationalen Team. Sie sprechen neben deutsch fließend englisch.

Ihre Aufgaben:
Im Rahmen des von der DFG-geförderten Projekts „Functional characterization of epigenetically regulated zinc finger proteins important for malaria parasite transmission“ werden Sie die molekulare Grundlagen der epigenetisch kontrollierten Genregulation in den Sexualstadien von P. falciparum untersuchen und dabei die Rolle von ausgewählten Zinkfingerproteinen während den Entwicklungsvorgängen des Parasiten beleuchten. Das Projekt beinhaltet gentechnische Arbeiten der Sicherheitsstufe 2 und schließt Zellkulturarbeiten, rekombinante DNA-Techniken, immunbiochemische Methoden, Proteinexpression und die Herstellung von Antikörpern in tierischen Systemen sowie mikroskopische Arbeiten mit ein.

Unser Angebot:
Die Stelle ist ab dem 1. Mai 2019 zu besetzen. Die Einstellung erfolgt im Beschäftigtenverhältnis. Die Eingruppierung richtet sich nach dem TV-L (E13 50%). Die persönlichen Voraussetzungen müssen erfüllt sein. Die Möglichkeit zur Promotion ist gegeben.

Für Vorabinformationen steht Ihnen
Herr Dr. Che Julius Ngwa
Tel.: +49 (0) 241-80-20838
E-Mail: Diese E-Mail-Adresse ist gegen Spambots geschützt! JavaScript muss aktiviert werden, damit sie angezeigt werden kann.
http://www.zai.rwth-aachen.de

Ihre Bewerbung richten Sie bitte bis zum 15.02.2019 per E-Mail an Herrn Dr. Che Julius Ngwa.

 

 


 

 

A PhD position is available at the Department of Parasitology, Center of Infectious Diseases, University of Heidelberg Medical School.


PhD in molecular Parasitology and Cell biology

Introduction: Our group works on the interaction of the malaria parasite with its chosen host cell, the mature human erythrocyte. The group is funded by the German Research Foundation, and currently composes four PhD student, the group leader (Jude Przyborski) and rotating bachelor/master and medical students. We are situated in the Department of Parasitology at the University of Heidelberg Medical School. The Department consists of 2 Professors and 8 groups which all work on the biology of the malaria parasite, and we are thus ideally situated to offer an intellectually stimulating and supportive scientific environment. Heidelberg itself is a lovely town (especially in the summer), and is home to the oldest and one of the most respected Universities in Germany (https://www.klinikum.uni-heidelberg.de/Malaria-9-Przyborski.143168.0.html).

Additional training opportunities: The selected student will be encouraged to enter the HBIGS graduate school programme which offers numerous opportunities for additional training (http://www.hbigs.uni-heidelberg.de).

Project Title: Atypical protein transport in malaria-infected erythrocytes: How does a membrane protein “jump” across membranes?

Project leader: PD Jude Przyborski

Application Deadline: 28 February 2019

Start of PhD project: ASAP

Source of Funding: DFG

Level of funding: Funding is available at E13/65% level for 3 years

Application: Please send your CV, statement of motivation and contact details for three referees by email to Diese E-Mail-Adresse ist gegen Spambots geschützt! JavaScript muss aktiviert werden, damit sie angezeigt werden kann. . Alternatively, you can apply via the HBIGS online system (http://www.hbigs.uni-heidelberg.de).

Project Description: Malaria is still one of the most important infectious diseases infecting humans, and kills about 400, 000 people annually. For this reason, it is essential to understand more about the cell and molecular biology of this parasite. Earlier studies on the basic cell biology of this parasite have revealed a multitude of novel phenomenon, including many related to how the parasite traffics proteins to various structures (Przyborski et al, 2016). This project aims to address one particular phenomenon, the transport of proteins beyond your boundary of the parasite’s plasma membrane. A previous study from our lab has uncovered an unusual pathway for trafficking of parasite proteins in this way, and this current project aims to build on our experience and further understand this process (Tribensky et al, 2017).

The human malaria parasite P. falciparum invades and propagates within mature red bloods cells. During invasion, the parasite envelops itself within a so-called parasitophorous vacuolar membrane (PVM), which acts as an interface between parasite and host cell, and is implicated in a number of processes important for parasite propagation. To this end, the parasite synthesises a number of proteins which intercalate into the PVM and are essential for parasite survival. However, it is so far unclear how exactly these proteins are both trafficked and sorted from the parasite to the PVM. In this project, using a number of complementary techniques, we aim to address this question and elucidate both the signals and mechanisms utilised to enable protein transport to the PVM. As a model protein, we shall firstly dissect the sorting signals and mechanisms required for the transport of the Exported Protein 1 to the PVM. Principles uncovered during this initial study will then be used to study the trafficking of further PVM-resident proteins such as members of the ETRAMP family. As protein transport to the PVM is a process underlying intra-erythrocytic development of the parasite and the subsequent pathology in the human host, any novel pathways may prove to be a chink in the parasite’s armour which may later be targeted for development of anti-parasitic drugs.

References:

Przyborski, J.M., Nyboer, B. & Lanzer, M. (2016) Ticket to ride: export of proteins to the Plasmodium falciparum-infected erythrocyte. Mol Microbiol, 101, 1-11.

Tribensky, A., Graf, A.W., Diehl, M., Fleck, W. & Przyborski, J.M. (2016) Trafficking of PfExp1 to the parasitophorous vacuolar membrane of Plasmodium falciparum is independent of protein folding and the PTEX translocon. Cell Microbiol, 19

Methods that will be used:

Molecular biology, protein biochemistry, proteomics, cell culture, transfection, cell-based assays.

Profile of candidate’s qualification:

Candidates should have a masters degree in molecular cell biology, biochemistry or equivalent disciplines. Experience with cell culture, protein biochemistry and proteomics would be an advantage. In addition, excellent knowledge in English (speaking, reading, writing) is required. The candidate should be able to independently solve problems and have a willingness to learn new techniques, including culture and transfection of malaria parasites, cell-based assays and protein purification.

Keywords:

Malaria, protein transport, erythrocytes, membrane transport.

 

 


 

 

 

 

Zuletzt aktualisiert am Donnerstag, den 31. Januar 2019 um 07:07 Uhr